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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in many tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are explained. Two further splice variants were described but thorough nucleotide sequence info is not really nevertheless available.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally includes the epitope acknowledged with the antibody.

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This guide outlines further more attainable optimization of mobile and immunoassay parameters to be certain the very best results are...

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This guideline outlines even more doable optimization of cellular and immunoassay parameters to be sure the very best final results are...

Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from your blocked antibody as opposed to the antibody on your own, one can see which staining is particular.

Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation styles which add to your temporal coordination of every mitotic occasion. Cyclin E sorts a complex with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates at the G1-S stage boundary which is degraded as cells development through S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...

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